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Confocal microscope: Zeiss Meta 510

 

Excitation wavelengths: 458, 488, 514, 543, 633nm
HFT: 488; 458/514; 458/543; 477/543; 488/543; 514/633
HFT UV: 488/543/633
NFT: 515, 545, 570, 635VIS
LP: 475, 505, 530, 560, 585, 650
BP: 475-525; 505-530; 505-550; 530-600; 560-615; 585-615



Confocal mikroskope: Leica SP8 (DM6 CS, aufrecht)

  • bright field detector
  • motorised stage, stitching
detectors1xPMT, 3xHyD
fluorescence filter
  • DAPI (emission:BP470/40);
  • L5 (emission:BP527/30);
  • Rhodamin (emission: BP585/40)
laser lines/excitation wavelengths405nm, 488nm, 552nm, 638nm
deconvolutionHyVolution2
lenses
  • PL APO 10x/0.40;
  • PL APO 20x/0.75 IMM, CorrR (water,glycerin,oil);
  • FLUOTAR L 25x/0.95 W (water immersion);
  • PL APO 63x/1.30 Glyc. CorrR (glycerin immersion);
  • PL APO 63/1.2 W Corr. (water immersion)
Notch filters (excitation)445, 488, 514, 448/514, 488/552/638, 594, 445/594


 

Delta Vision RT (ChromaPhor)

 

Type   Widefield Fluorescence Mikroskop mit rekonstruktive Dekonvolution
Model    Inverse, IX71 Olympus
Objektive   
  • UPlan-flu 10x
  • UPlanSApo 20x
  • NeuFluor 25x Oil/W/G
  • UApo 40x Oil DIC
  • PlanApoN 60x Oil
  • UPlanSApo 100x Oil
Camera    Coolsnap HQ (Photometrics)
Filter    Standard set Dapi, FITC, TRITC, Cy 5, Live-cell set CFP, GFP, YFP, DsRed
Light source    Hal 100, HG
Chamber   
Heating Unit XL
Software   
softWorX
Best used for    z-stack, time-lapse, tile-scan, multipoint, reconstructive deconvolution, ...